Specificity Changes in the Evolution of Type II Restriction Endonucleases
نویسندگان
چکیده
منابع مشابه
Specificity changes in the evolution of type II restriction endonucleases: a biochemical and bioinformatic analysis of restriction enzymes that recognize unrelated sequences.
How restriction enzymes with their different specificities and mode of cleavage evolved has been a long standing question in evolutionary biology. We have recently shown that several Type II restriction endonucleases, namely SsoII (downward arrow CCNGG), PspGI (downward arrow CCWGG), Eco-RII (downward arrow CCWGG), NgoMIV (G downward arrow CCGGC), and Cfr10I (R downward arrow CCGGY), which reco...
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The endonucleases from the Type IIB restriction-modification systems differ from all other restriction enzymes. The Type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex. The fact that ...
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In the past seven to eight years we have witnessed the development of a new DNA technology that has fundamentally altered our approach to modern genetics. The basic ingredients of this new technology are the cleavage-site-specific restriction enzymes: a special class of bacterial endonucleases that can recognize specific nucleotide sequences in duplex DNA and produce double-stranded cleavages. ...
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In the past seven to eight years we have witnessed the development of a new DNA technology that has fundamentally altered our approach to modern genetics. The basic ingredients of this new technology are the cleavage-site-specific restriction enzymes: a special class of bacterial endonucleases that can recognize specific nucleotide sequences in duplex DNA and produce double-stranded cleavages. ...
متن کاملStructure and function of type II restriction endonucleases.
More than 3000 type II restriction endonucleases have been discovered. They recognize short, usually palindromic, sequences of 4-8 bp and, in the presence of Mg(2+), cleave the DNA within or in close proximity to the recognition sequence. The orthodox type II enzymes are homodimers which recognize palindromic sites. Depending on particular features subtypes are classified. All structures of res...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2005
ISSN: 0021-9258
DOI: 10.1074/jbc.m409020200